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This Getting Started guide provides tutorials to familiarize you with BD FACSDiva™ software. If you are a new user, use the tutorials to get started with the software. If you are an experienced user, try the updated tutorials to become familiar with new software features. Updated features are highlighted in the New Features section. New Features University of Helsinki Performance. With superior optics and fluidics for maximized sensitivity and resolution, the BD LSRFortessa™ helps you analyze your sample with ease.

difluoride (PVDF) membrane (Amersham Biosciences, Arlington Heights, IL). Exempelvis möjliggör HTS-teknologier med hög genomströmningssekvens och bias still remained obscure, but could be due to use of the solution PCR method. Our study will help researchers improve the design of future SELEX strategies for flow cytometry (BD Fortessa, Analytical Cytometry Core, City of Hope, CA). Djurförsök; Ytterligare information; Kompletterande information; PDF-filer (CHO-K1, ATCC: CCL-61) odlades i ChoMaster HTS (Cell Culture Technologies GmbH, analyserades med användning av en LSRII Fortessa flödescytometer (Becton med användning av FACSDiva (BD Biosciences, Franklin Lakes, NJ, USA, Ladda ner PDF Cellfärgning analyserades på en LSR Fortessa (BD Biosciences), och resultaten bearbetades med användning av FlowJo (Treestar, Ashland, BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with samples using the HTS, consult the appropriate cytometer user’s guide for information about flow cytometer operation, daily shutdown, maintenance, and troubleshooting. The BD High Throughput Sampler User’s Guide assumes you have a working knowledge of basic Microsoft® Windows® operation. If you are not familiar with the Windows operating FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no Title: Microsoft Word - Stanford Fortessa HTS User Guide.docx Author: lovelace Created Date: 8/14/2013 10:44:41 PM Performance without peer, choice without compromise The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency.

Fortessa FILTERconfig - Lund Stem Cell Center

This will warm up the lasers and pressurize the fluidics. 2.

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Turn on main power switch (Green button-right side) 4.

Check the fluidics. Fludics Default cytometer configuration for the LSR Fortessa analyzer: For more information click the following links: BD LSR Fortessa user manual.
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For the BD LSR II, the 12 Jan 2019 DETAILED INFO AND PHOTOS FOR THIS CAN BE FOUND ON OUR WEBSITE AT For authorization to use the FACSCanto II cytometer, you first have to receive a training and must accept the user rules of the facility!

2. Switch on the Computer and login to the DiVa software. Even if you are using tubes instead of the HTS, you will have to wait for the HTS to go through its prime. 3.
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•. BD FACSDiva Software HTS module for plate analysis (opera%ng procedure in separate guide). See Appendix Start the BD FACSDiva software program by double-‐clicking on the icon on in the dropdown menu under “User Name”, filling in your.

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If you need to switch the configuration to acquire your samples in FACS tubes, please follow these steps: BD High Throughput sampler. The HTS supports standard 96 (u bottom plates best) and 384 well plates. It is operated using DiVA allowing users to define customized delivery protocols, mixing, wash and analysis parameters If you have the BD HTS option, connect the HTS sample coupler to the SIT and close the HTS door. Refill fluids and empty waste, if needed, and then select Cytometer > Long Clean . When the long clean is complete, run a fluidics shutdown and turn off the system.

It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: … FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn … to)running)an)HTS)experiment.)The)HTS)uses)more)sheath)fluid)than)loading) thetubes.) 2. Switch)the)acquisition)control)switch)to)plate)mode.) 3. Remove)the)tube)of)DI)water)fromthe)SIT.) 4. Attach)the)HTS… Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates. In high- throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes. Page 2 Cleaning Procedure after Measurement Include 3x4 BD FACS LSRFortessa HTS Operation Manual 5 • Select throughput mode by clicking the corresponding mode button in the Setup view.